One of the most significant ornamental, medicinal plants in the world is sage (Salvia offecialis L.). It is susceptible to a wide range of diseases, including root rot brought on by pathogens including Macrophomina phaseoli, Rhizoctonia solani, Fusarium solani, and others that have been identified by multiple studies. Due to the non-traditional nature of the crop, root rot is a serious disease that results in significant loss and little is known about the relationship between the pathogen(s) and its management. Therefore, the current inquiry was conducted to identify the pathogen(s) responsible for the root rot/wilt symptoms and to test if UV-C application may be used to treat this disease. It has been shown that the disease is spread throughout all trial fields in the several districts of the Minia Governorate. Twenty three isolates of fungi, belonging to seven different genera, were obtained from naturally infected samples of sage. The most dominant fungi were Fusarium solani, M. phasiolina, Rhizoctonia solani, which have proved their ability to infect sage plants inducing root rot/ wilt symptoms. The mycelial growth of these fungi was reduced gradually when exposed to UV-C radiation, the highest inhibition was recorded when mycelium was exposed to to UV-C radiation for 120 seconds. Immersed cuttings of sage in pre-treated mycelium of these fungi with UV-C led to reduce the percentages of disease incidence and severity. Also, when cuttings of sage were exposed to UV-C for different periods of exposure, the disease incidence and severity were reduced more than mycelial treatment.