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QUANTITATIVE DETECTION OF ADULTERATION OF VARIOUS PROCESSED MEAT PRODUCTS WITH SOYBEAN PROTEIN BASED ON DIFFERENT HISTOLOGICAL METHODS

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Histologyand cytology

Abstract

Egypt's meat producers produce a variety of meat brands that used soybean proteins because of high meat pricing. Allergens have been discovered in products that were not declared allergen-free. A total of 540 samples were obtained at random from various food supermarkets in Sohag City. Using haematoxylin and eosin and various histochemical dyes, light microscopy was utilized to identify the structural properties of the soybeans. The structure of all components was determined using fluorescent microscopy and acridine orange dye. Soybeans were discovered in meat samples and the cotyledon's vascular tissue and parenchymal cells rich in pectin confirmed this discovery by immunohistochemistry techniques. After immunohistochemical confirmation with an antirabbit soybean marker, the proportion of soybean was determined using histological procedures and image analysis. The percentage of soybeans in various meat products, including minced meat from two quality levels, sausages from two quality levels, raw kofta, and beef burgers from two grades, as well as chicken and luncheon meats, was 85%, 90%, 64%, 76%, 80%, 65%, 97%, 82%, and 69%, respectively. Soybean percentages in the examined samples did not fulfill Egyptian standards for soya percentages, which is approximately 10%, and this may affect consumers' health. In conclusion, all tested meat samples contained a high percentage of soya adulteration, necessitating increased supervisory to reduce meat fraud.

DOI

10.21608/avmj.2024.286152.1249

Keywords

immunohistochemical stain, scanning electron microscope and fluorescent microscope, light microscopy, Soybean, meat products

Authors

First Name

HANAN

Last Name

ABD-ELHAFEEZ

MiddleName

H.

Affiliation

Department of Cells and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

Email

hhnnzz91@aun.edu.eg

City

-

Orcid

0000-0002-2547-0709

First Name

RANIA

Last Name

ZAKI

MiddleName

SAMIR

Affiliation

Faculty of Veterinary Medicine, New Valley University, Meat Hygiene.

Email

raniasamir@vet.nvu.edu.eg

City

-

Orcid

-

First Name

ABDELRAHEIM

Last Name

ATTAAI

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt. Department of Anatomy and Histology, School of Veterinary Medicine, Badr University in Assiut, Assiut, Egypt.

Email

abdelraheim.attaai@aun.edu.eg

City

Assiut

Orcid

0000-0001-8481-2687

First Name

ABEERA

Last Name

EL-SAYED

MiddleName

MOHAMOUD

Affiliation

Faculty of Medicine, Sohag University Hospital, Sohag University, Sohag, Egypt.

Email

abeeramahdy2019@yahoo.com

City

sohag

Orcid

-

First Name

MOHAMMED

Last Name

ALI

MiddleName

A.

Affiliation

Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.

Email

mohali@aun.edu.eg

City

-

Orcid

-

First Name

SOHA

Last Name

SOLIMAN

MiddleName

A.

Affiliation

Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.

Email

soha_soliman@vet.svu.edu.eg

City

-

Orcid

-

First Name

DOAA

Last Name

ABD EL-MAGEED

MiddleName

SAWFAT

Affiliation

8 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, New Valley University, Egypt.

Email

safwat_doaa79@yahoo.com

City

-

Orcid

-

Volume

70

Article Issue

182

Related Issue

48831

Issue Date

2024-07-01

Receive Date

2024-04-30

Publish Date

2024-07-01

Page Start

136

Page End

159

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_363601.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=363601

Order

12

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

QUANTITATIVE DETECTION OF ADULTERATION OF VARIOUS PROCESSED MEAT PRODUCTS WITH SOYBEAN PROTEIN BASED ON DIFFERENT HISTOLOGICAL METHODS

Details

Type

Article

Created At

28 Dec 2024