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363312

DETECTION OF MORGANELLA MORGANII IN FROZEN MACKEREL VIA MULTIPLEX PCR RECOGNITION AND ASSIMILATION

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Food biotechnology

Abstract

Morganella morganii is a psychrotolerant organism recognized as a histamine-producing bacteria by decarboxylation of histidine amino acid, it has been involved in cases of histamine fish poisoning reported in different seafood products. In this study, we collected a total of 125 random samples of frozen Mackerel fish stored at -18°C from different fish markets in New Valley Governorate, Egypt. Through isolation from the tissue of fish samples on Chromogenic agar plates, bacteria forming light brown colonies were identified. Confirmatory identification of bacteria was conducted using 16S rRNA as a species-specific gene, revealing the presence of 14 M. morganii strains from samples, representing an incidence of 11.2% of all samples. Morganella morganii is a prominent adaptive nosocomial bacterium that can cause urinary tract infections and bacteremia.

DOI

10.21608/avmj.2024.291465.1265

Keywords

Morganella morganii, mackerel fish, Histamine fish poisoning, PCR

Authors

First Name

ESRAA

Last Name

MOHAMED

MiddleName

MARZOUK

Affiliation

Department of Food Hygiene, Safety and Technology (Meat Hygiene), Faculty of Veterinary Medicine, New Valley University, Egypt.

Email

esraamarzouk67@gmail.com

City

New Valley

Orcid

-

First Name

ASHRAF

Last Name

ABD-EL-MALEK

MiddleName

MOHAMED

Affiliation

Department of Food Hygiene, Safety and Technology, Faculty of Veterinary Medicine, Assiut University

Email

ashraf2015@aun.edu.eg

City

Assiut

Orcid

0000-0002-0586-0777

First Name

WALEED

Last Name

ALI

MiddleName

SENOSY

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, New Valley University, Egypt.

Email

senosy_76@yahoo.com

City

-

Orcid

-

First Name

RANIA

Last Name

ZAKI

MiddleName

SAMIR

Affiliation

Department of Food Hygiene, Safety and Technology (Meat Hygiene), Faculty of Veterinary Medicine, New Valley University, Egypt.

Email

raniasamir5555@gmail.com

City

-

Orcid

-

Volume

70

Article Issue

182

Related Issue

48831

Issue Date

2024-07-01

Receive Date

2024-05-21

Publish Date

2024-07-01

Page Start

106

Page End

113

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_363312.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=363312

Order

9

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

DETECTION OF MORGANELLA MORGANII IN FROZEN MACKEREL VIA MULTIPLEX PCR RECOGNITION AND ASSIMILATION

Details

Type

Article

Created At

28 Dec 2024