Beta
332384

MOLECULAR CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA ISOLATED FROM SOME FERMENTED MEAT PRODUCTS

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Meat hygiene & meat by-products

Abstract

In this investigation, 123 lactic acid bacterial strains (LAB) were isolated and identified from 30 of each type of fermented meat products (sausage, salami and hotdog). Colony and cell morphology, Gram reaction, biochemical assessments and sequencing of the 16S rRNA gene using PCR technique were principally employed to identify some of the LAB strains. According to the molecular characterization data, Lactobacillus plantarum, Lactococcus lactis spp. Lactis and Leuconostoc mesentroides spp. mesentroides were the three most common LABs.  Additionally, the results revealed that the mean inhibition zone of antibacterial activity of pure bacteriocin of LAB was expressed as 21.50±3.55, 25.71±2.18, 20.07±5.16, and 2.93±3.97 in millimeter against Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 and Salmonella Typhimurium ATCC 14028, respectively. So, pure bacteriocin is shown by its capability to inhibit the growth of both Gram-positive and Gram-negative bacteria. Currently, bacteriocins producing LAB are frequently utilized as an alternative to antibiotics in the food sector as a food preservation agent. In terms of promoting food safety, extending shelf life, and upgrading product quality, it will further expand the application area of bacteriocin. In the categories of fermented food sectors, they are economically very important.

DOI

10.21608/avmj.2023.230141.1178

Keywords

Fermented meat products, lactic acid bacteria, PCR, bacteriocin

Authors

First Name

SAMAR

Last Name

AHMED

MiddleName

MAHMOUD

Affiliation

Department of Food Hygiene, Veterinary Medical Sciences, Assiut University, Assiut, Egypt

Email

samarmahmoud402@gmail.com

City

-

Orcid

29104042501289

First Name

DOAA

Last Name

ABD EL-AZIZ

MiddleName

MOHAMED

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

Email

doaassiut@yahoo.com

City

Assiut

Orcid

-

First Name

NAEIMA

Last Name

HAMMAM YOUSEF

MiddleName

MOHAMED

Affiliation

Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut , Egypt

Email

naeima@aun.edu.eg

City

Assiut

Orcid

0000-0002-6327-141×

Volume

70

Article Issue

180

Related Issue

45134

Issue Date

2024-01-01

Receive Date

2023-08-26

Publish Date

2024-01-01

Page Start

110

Page End

122

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_332384.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=332384

Order

10

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA ISOLATED FROM SOME FERMENTED MEAT PRODUCTS

Details

Type

Article

Created At

28 Dec 2024