The Hepatic disease all over the world had a more attention towards the prevention methods; planned diet can effective and protective. Therefore, this study was aimed to examine the antioxidant and hepatoprotective role of the dried savory leaves (1 and 2%) and its dried hydroethanolic extract (25 and 50 ppm) against carbon tetrachloride (CCl4) induced liver damage in rats. Also, the effect of savory leaves extract on biofilm formation of some microbial strains was studied. Liver injury group revealed significant increase in serum liver parameters such as AST, ALT and ALP activities and malondialdehyde (MDA) levels. Whereas, reduction in superoxide dismutase (SOD) activity was done. Furthermore, the liver injury rats recorded hepatic histological several and severe alteration due to hepatocellular damage by CCl4. Treatments by the dried savory leaves and its extract at different levels resulted significantly improved the liver function and oxidative stress parameters; also, suppresses the histological alteration in liver. Both Gram-positive and Gram-negative bacteria have the ability to form biofilm with varying degrees. The savory ethanolic extract has an antibiofilm effect against the studied bacteria strains. Also, savory leaves extract exhibited scavenging activity due to their containing from phenolic and flavonoids, responsible for their antioxidant, antibiofilm and hepatoprotective properties. It could be concluded that the dried savory leaves and their extracts could be used as ingredients in functional foods for hepatoprotective.