Salmonella is a serious organism in the commercial poultry industry. Eggs contaminated with Salmonella have been internationally significant sources of human illness for several decades. Most egg-associated illness has been attributed to Salmonella enteritidis, but a few other serovars (notably S. heidelberg and S. typhimurium) are also sometimes implicated. This study for isolation and identification of Salmonella from 5 Layers farms in El-Menofia Governorate by cultural and Molecular methods. The overall prevalence of Salmonella spp. was 15% (n=15/100). 6 Salmonella isolates were found from egg samples (4 isolates from eggshell, 2 isolates from egg contents).  9 isolates from liver, egg follicle, intestine and cloacal swaps were detected. The percentage of S. kentucky, S. typhimurium, S. infantis and S. enteritidis were 33.3%, 26.7%, 20% & 20%, respectively. The highest serotype isolated was S. kentucky (33.3%). Aerobic plate count was performed for the 60 eggshells (before the treatment as control), then 20 eggshells were treated with slightly acidic electrolyzed water, 20 eggshells were treated with slightly alkaline electrolyzed water and 20 eggshells treated with slightly acidic electrolyzed water then slightly alkaline water, respectively. Decontamination of eggs with slightly acidic electrolyzed water(SAEW) showed higher bactericidal effect compared to slightly alkaline (SAIEW). The highly effect obtained when using the acidic and alkaline water together. Therefore, decontamination of the egg surface would be a critical step in improving the microbiological safety and to extend the shelf life of the eggs used for human consumption.