The capability of pathogenic shiga toxin-producing E. coli (STEC) to produce the toxins Stx1 and Stx2, which cause intestinal disorders, makes their presence in dairy products a public health problem. This study major goal was to identify the presence of ESBL-producing E. coli and shiga toxins in several cheese varieties that are sold in and around Assiut City. Ninety random samples of white soft cheese, including Kariesh, Domiati, and Tallaga cheese (30 of each), were collected and checked for the presence of E. coli, which were 86.7, 0.0, and 16.7%, respectively. A total of 31 E. coli isolates (34.4%)  were recovered from 90 samples. Twelve E. coli isolates were positive for ESBL production and were multidrug-resistant. Ten of which were molecularly examined for the detection of shiga toxins (Stx1 and Stx2) genes and a fliC-specific gene for E. coli O175:H7. Two isolates harbored Stx1, four isolates harbored Stx2 virulence gene, and seven isolates were E. coli O175:H7. The susceptibility to ten antimicrobial agents was evaluated by the disk diffusion method. The results of antimicrobial susceptability testing showed high sensitivity to colistin (CL), Ciprofloxacin (CP), trimethoprim-sulfamethoxazole (COT), gentamicin (GEN), and tetracycline (TE) with 90.3, 87.1, 83.8, 80.6, and 58.1%, respectively,while resistance to amoxicillin (AMX), Erythromycin (E), ampicillin (AMP), cefotaxime (CTX), and amoxicillin-clavulanic acid (AMC) with 93.6, 93.6, 77.5,51.5 and 48.4%, respectively. The acquired initial findings revealed sanitary and hygienic deficiencies throughout the production process of Kariesh cheese and showed that these products able to contain multidrug-resistant and virulent strains of E. coli and may play a role in the spread of antimicrobial- resistant (AMR) to other microorganisms, posing a risk to public health. This implies that soft cheese needs to be viewed as a medium through which possibly harmful germs can spread. To get thorough findings, more research and analysis of more samples are still required..