A total of 90 random samples of meat products represented by minced fresh beef, beef burger and kofta (30 of each) were collected from various markets in Kalyobia governorate, Egypt to assess their bacteriological profile. The average values of APC were 4.46 ± 0.09, 4.69 ± 0.11 and 4.78 ± 0.26 (log cfu /g) in examined minced fresh beef, beef burger and kofta samples, respectively. Moreover, Coliforms counts ranged from 2.48 to 3.95 with an average 3.41± 0.08 (log cfu/g) in minced fresh beef, 2.6 to 4.81 (3.6 ± 0.12 log cfu /g) in beef burger and 2.69 to 4.89 (3.92 ± 0.28 log cfu /g) in kofta. The mean values of Staph. aureus count (log cfu /g) in examined samples of minced, beef burger and kofta were 2.38 ± 0.06, 2.89 ± 0.07 and 2.68 ± 0.11, respectively. Furthermore, the incidence of different enteropathogenic serotypes of E. coli isolated from minced fresh beef were, O₂₆: H₁₁ EHEC (6.7%), O₉₁: H₂₁ EHEC (3.3%) and O₁₂₇: H₆ ETEC (3.3%). While, O₉₁:H₂₁ EHEC (3.33%), O₁₁₁:H₂ ETEC (6.67%), O₁₂₈:H₂ ETEC (3.3%), O₈₆ EPEC (6.7%) and O₁₂₁:H₇ ETEC (3.3%) serotypes isolated from beef burger samples. Moreover, O₂₆: H₁₁ EHEC (3.3%), O₁₁₄:H₄ EPEC (3.3%), O₁₅₉ EIEC (3.3%), O₅₅:H₇ EPEC (3.3%), O₁₁₁:H₂ ETEC (3.3%), O₁₄₆:H₂₁ EPEC (3.3%) and O₁₂₁:H₇ ETEC (3.3%) isolated from kofta samples. The serological identification of Salmonella isolates revealed the detection of (3.3%) S.Enteritidis in minced fresh beef and kofta and (3.3%) of S.Infantis in minced fresh beef only while, prevalent serotype was S.Typhimurium, as (3.3%) isolated from minced fresh beef and beef burger and (6.7%) from kofta samples. Also, (3.3%) S.Virchow and S.Tsevie were isolated from beef burger samples. The public health importance of the isolated microorganisms and the recommended points were discussed. In addition, the evaluation of the efficacy of zinc oxide nanoparticles in improving minced fresh beef quality, while being in cold storage and determination of the antibacterial and antioxidant activities of ZnO nanoparticles. Using various concentrations (20, 40, and 60 ppm) of Zinc oxide ZnO NPs. Treated groups with ZnO nanoparticles showed a reduction in the inoculated E. coli.