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349915

Prevalence and bacteriological investigation of Bacillus cereus isolated from meat and milk products in El-Gharbia governorate, Egypt.

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Microbiology

Abstract

Bacillus cereus is a food-borne pathogen that can cause emesis, diarrhea and spoilage of different food products. For this, 125 random samples of meat products (luncheon and sausage) and milk products (ras or romy cheese, karish cheese and rice pudding), 25 of each, were taken from various shops and marketplaces in El-Gharbia governorate, Egypt to be examined for prevalence, isolation and identification of Bacillus cereus. Additionally, the significance of Bacillus cereus for public health was covered. The bacteriological examination of collected samples showed that, a total of 40/125 (32%) Bacillus cereus strains were obtained; mostly they were isolated from Ras cheese 14/25 (56%) followed by Luncheon 11/25 (44%), Rice pudding 8/25 (32%) then from Sausage 7/25 (28%) and there is no isolated Bacillus cereus from Karish cheese 0/25 (0%). Polymerase chain reaction for five random isolated studied strains showed that groEL (diagnostic and marker gene of Bacillus cereus) gene was detected in all five studied strains. The anti-microbial sensitivity test for 20 Bacillus cereus strains revealed that they were completely sensitive to ciprofloxacin, norfloxacin and levofloxacin (100%) followed by gentamycin (80%), doxycycline (60%), chloromphenicol (50%), erythromycin (30%) then clindamycin (20%), whereas they were completely resistant to ampicillin, cefipime, aztreonam and amoxicillin-clavulanic acid.

DOI

10.21608/bvmj.2024.260058.1770

Keywords

Bacillus cereus, meat and milk product, antimicrobial sensitivity, PCR, groEL

Authors

First Name

Shimaa

Last Name

Elhaw

MiddleName

Ibrahim Mohamed rashad

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Egypt.

Email

shimaaelhaw89@gmail.com

City

Tanta, Gharbia

Orcid

-

First Name

Seham

Last Name

Homouda

MiddleName

Nazeeh

Affiliation

Department of Food Hygiene, Animal Health Research institute (AHRI) Tanta Branch, Agricultural Research Center (ARC), Egypt.

Email

seham.hamouda@yahoo.com

City

Tanta

Orcid

-

First Name

Ashraf

Last Name

Abd El-tawab

MiddleName

Awad

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Egypt.

Email

ashraf.awad@fvtm.bu.edu.eg

City

Toukh

Orcid

0000-0002-1221-3340

Volume

46

Article Issue

1

Related Issue

47107

Issue Date

2024-04-01

Receive Date

2024-01-09

Publish Date

2024-04-01

Page Start

125

Page End

129

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_349915.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=349915

Order

23

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Prevalence and bacteriological investigation of Bacillus cereus isolated from meat and milk products in El-Gharbia governorate, Egypt.

Details

Type

Article

Created At

24 Dec 2024