This study was conducted to monitor the prevalence of Vibrio spp. in some fresh fish and Shrimp. A total of 90 samples of Oreochromis niloticus, Mugil cephalus, and penacus japonicas (30 samples of each) which were collected from from different markets in Qalyubia governorate and examined for the presence of Vibrio spp. and its serotypes. The incidence of Vibrio spp. was 36.67%, 30%, and 80% for Oreochromis niloticus, Mugil cephalus, and penacus japonicas respectively. Subsequently, Oreochromis niloticus samples recorded 16.67%, 13.33%, 3.33%, and 3.33% for V. mimicus, V. parahaemolyticus, V. alginolyticus, and V. vulnificus, respectively. For Mugil cephalus samples, the results were 3.33%, 10%, and 6.67% for V. mimicus, V. parahaemolyticus, and V. alginolyticus, respectively. At the same time, penacus japonicas samples illustrated 10%, 33.33%, 23.33%, 10%, and 3.33% for V. mimicus, V. parahaemolyticus, V. alginolyticus, V. vulnificus, and V. cholera respectively. All isolates of V. parahaemolyticus (17 isolates) showed resistance to erythromycin at a percentage of 100%, while most of them (94.1%) were sensitive to amikacin with an average MAR index of 0.457.