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365296

Mycological evaluation of some ready to eat meat products with special reference to molecular characterization

Article

Last updated: 23 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Food safety / meat quality

Abstract

Sixty samples of random meat products luncheon, basterma and hawawshi (20 of each) were collected from different localities In Kalyobia governorate, Egypt. The collected samples were subjected to mycological examination, detection of aflatoxin B1 residues as well as the ability of isolated A.flavus for production of aflatoxin By and identification of toxigenic strains by PCR. The obtained results revealed that the examined luncheon samples had the highest mould count with a mean value 4.7x102 ± 2.1x102 followed by basterma samples 3.4x102±8.6x10 and Hawawshi samples had lower mould count 7.9 x10 ± 5.2 x10. Six mould genera could be detected and identified. The identified mould genera were belonging to genus Aspergillus, Penicillium, Cladosporium, Mucor, Eupenicillium and Talaromyces. The highest incidence of isolate among Aspergillus spp. was A. niger followed by A.flavus and A.parasiticus. The average concentration of aflatoxin B1 (µg/kg) in luncheon, basterma and hawawshi were 1.4± 0.63, 0.8 ±0.47 and 0.7± 0.36, respectively. Toxigenic A.flavus were subjected to PCR identification. Three A.flavus toxigenic isolates were examined by polymerase chain reaction (PCR) with using specific primer (PEPOI &PEPO2). PCR products of A.flavus strains were positive on agarose gel electrophoresis of PCR amplification products showing 200 bp. The public health importance of the isolated moulds and aflatoxins and the recommended points were discussed.

DOI

10.21608/vmjg.2016.365296

Keywords

meat products, Mycotoxins, PCR, toxigenic strains

Authors

First Name

Shaltout

Last Name

A

MiddleName

-

Affiliation

Department of Food Control, Faculty of Vet. Med., Benha University, Egy pt

Email

-

City

-

Orcid

-

First Name

Salem

Last Name

M

MiddleName

-

Affiliation

Department of Food Control, Faculty of Vet. Med., Benha University, Egy pt

Email

-

City

-

Orcid

-

First Name

El-diasty

Last Name

E

MiddleName

-

Affiliation

Department of Food Control, Faculty of Vet. Med., Benha University, Egy pt

Email

-

City

-

Orcid

-

First Name

Fatema

Last Name

Diab

MiddleName

-

Affiliation

Department of Food Control, Faculty of Vet. Med., Benha University, Egy pt

Email

-

City

-

Orcid

-

Volume

62

Article Issue

3

Related Issue

47598

Issue Date

2016-07-01

Receive Date

2024-07-06

Publish Date

2016-07-01

Page Start

9

Page End

14

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 62_Issue 3_Pages 9-14.pdf

PDF . 2.8MB

Link

https://vmjg.journals.ekb.eg/article_365296.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=365296

Order

365,296

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Mycological evaluation of some ready to eat meat products with special reference to molecular characterization

Details

Type

Article

Created At

23 Dec 2024