Subjects
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Food safety / meat quality
Abstract
Enterotoxigenic Staph. aureus is one of the causative agents of food borne intoxication. For this reason, determination of its prevalence in foods is important with respect to assessing public health risk. Many different types of foods are considered as a source of the bacterium and have been identified as a potential source of shiga Toxin-producing Escherichia coli. The research study focused on detection of the virulent genes of Staph. aureus enterotoxins and shiga toxins of E. coli in fresh and frozen chicken and ready- to- eat chicken meat products (RTE), by using Multiplex PCR (m- PCR) technique. It was carried out on 20 isolates of Staph. aureus from different ready- to- eat chicken meat products (14 isolates) as well as fresh and frozen chicken carcasses (six isolates; three of each), the (Sea) gene of Staph. aureus had the highest prevalence of virulence genes. Shiga toxin] (Stx1) gene could be detected only in one strain (50%) isolated from both of chicken shawerma and chicken salad. Shiga toxin2(Stx2) could be detected in two isolates of E. coli from chicken Fajitas and chicken salad. The public health significance of both toxins was discussed.
DOI
10.21608/vmjg.2017.354157
Keywords
Ready to eat, enterotoxins producing genes, m-PCR, Staph. aureus, Shiga toxin
Authors
MiddleName
-Affiliation
Meat Hygiene and Control Department, Faculty of Veterinary Medicine, Cairo University
Email
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-Orcid
-MiddleName
-Affiliation
Food Hygiene Department, Animal Health Research Institute, Dokki-Giza
Email
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-Orcid
-MiddleName
-Affiliation
Meat Hygiene and Control Department, Faculty of Veterinary Medicine, Cairo University
Email
-City
-Orcid
-MiddleName
-Affiliation
Meat Hygiene and Control Department, Faculty of Veterinary Medicine, Cairo University
Email
-City
-Orcid
-Link
https://vmjg.journals.ekb.eg/article_354157.html
Detail API
https://vmjg.journals.ekb.eg/service?article_code=354157
Publication Title
Veterinary Medical Journal (Giza)
Publication Link
https://vmjg.journals.ekb.eg/
MainTitle
Detection of virulent genes of Staph aureus and E.coli in fresh, frozen chicken and Ready-to- Eat chicken meat products