A total of one hundred dog and cat food, fifty of each (25 dry and 25 canned) were analyzed for determination of their content of total aflatoxin using Enzyme linked Immunosorbent Assay (ELISA) and Staphylococcal enterotoxins using Set-RPLA. The mean value of total aflatoxin in dry food were 0.3212+ 0.02437 and 0.3068+0.01323 for dog and cat, but not
detected in all examined canned pet food samples as well as Staphylococcal enterotoxins. The level of positive samples compared with maximum legal limit set by Food and Drug Administration FDA (2001) 20 ug/kg. The public health significance of aflatoxin and measure of control were discussed