A total of fifty egg samples (25 of each of baladi and farm eggs) were collected from different markets in Cairo and Giza Governorates to assess their microbiological status and detection of antibiotic, pesticide and estradiol residues. The mean values of Aerobic Plate Count in baladi and farm eggs were 3.1×102 ±5.8×10 and 2×102 ±6.5×10 cfu/g respectively, which were within the limit recommended by EOS, 3169/2007 (2.5×104 cfu/g). The mean value of E. coli count in baladi eggs were 3×10 ±0.79×10 cfu/g which was higher than the limit recommended by EOS, 3169/2007 (10 cfu/g), while it has not been inferred in farm eggs. Salmonella species, Shigella and Clostridum perfrengens could not be detected in all examined samples. The mean values of yeast and mould count in baladi and farm eggs were 1.5×10 ±0.45×10
and 3.5×10 ±0.78×10 cfu/g respectively, which were compatible with the limit recommended by EOS, 3169/2007 (5×10 cfu/g). The presence of antibiotic residues in egg samples were detected qualitatively by using modified four plate test (FPT). Pesticide residues were determined with Agilent gas chromatograph (GC). Estradiol hormone levels were determined
wit Rida screen ELISA kit for tissue. The incidence of antibiotic residues in baladi and farm
eggs were 2 (8%) and 10 (40%), respectively. The mean concentration of heptachlor epoxide in baladi and farm eggs was 0.085±0.02 ppm, and was present in a percent of 8 (32%), while it could not be detected in examined farm eggs, above the MRL cited by Codex (2016) (0.05 mg/kg). Endosulfan couldn't detected in farm eggs and was present in baladi eggs in a
concentration of 0.105±0.003 ppm and incidence of 3(12%), above the MRL cited by Codex (2016) (0.03 mg/kg). Endrine couldn't be detected in baladi eggs and its mean value was 0.28±0.009 ppm in farm eggs and was present in a percent of 6(24%) above MRL cited by J F C R F (2016) (0.005 mg/kg). Dialdren not detected in baladi eggs but found in farm eggs
in a percent of 4(16%) with mean concentration of 0.25±0.03 ppm which above MRL recommended by Codex (2016) (0.1 mg/kg). Methoxychlor was present in both baladi and farm eggs in a concentration of 0.037±0.001 and 0.016±0.002 ppm with percent of 11(44%) and 6(24%) respectively, above MRL cited by J F C R F (2016) (0.01 mg/kg). Alfa-BHC, Gama BHC, Delta BHC, heptachlor, Aldrin, endosulfan 11, endosulfan sulfate, p,p-DDT, p,p-DDD, p,p -DDE and endrin aldehyde as well as organophosphorus pesticides couldn't be detected in both baladi and farm eggs. The mean value of estradiol in baladi and farm egg samples which were 0.030 ±0.001, 0.400±0.120 µg/kg respectively, within the permissible limit (1ppb) stated by Gracey (1986). All baladi egg samples are within acceptable daily intake (ADI) and 72% of farm eggs are within the acceptable daily intake (ADI) value for estradiol of 0.05 μg/kg body weight /day as assessed by JECFA (1999). The public health significance of the isolated organisms and harmful residues as well as recommended hygienic measures were discussed to ensure quality of eggs to safeguard the consumers.