One hundred fifty apparently healthy samples of some seafoods (30 each of Om EL-Khloul “Donax trunculus anatinus", baclawese “Tartufo di mare", boltifish “Oreochromis niloticus", sardine “Sardinella gibbosa" and cuttlefish “Sepia spp.") were randomly purchased during the period from May to August 2006 from Port-Said markets. The samples were examined for enumeration and isolation of halophilic Vibrio species as well as the incidence of Kanagawa positive phenomena in the isolated strains and their public health significance was determined. The incidence of positive samples for halophilic Vibrio species was 96.67% (29), 73.33% (22), 60.00% (18) 80.00% (24) and 70.00% (21) while the mean values of the total halophilic Vibrio counts were 8.3 X 103, 3.5 X 103, 1.4 X 103, 3.8 X 103 and 1.8 X 103 CFU/g of Om EL-Khloul “Donax trunculus anatinus", baclawese “Tartufo di mare", boltifish “Oreochromis niloticus", sardine “Sardinella gibbosa" and cuttlefish “Sepia spp." respectively. The numbers of halophilic Vibrio isolates were 69, 52, 41, 59 and 50 while the incidence of Kanagawa positive phenomena in these isolates was 13.04% (9), 9.62% (5), 4.88% (2), 8.47% (5) and 4.00% (2) in the examined samples of Om EL-Khloul “Donax trunculus anatinus", baclawese “Tartufo di mare", boltifish “Oreochromis niloticus", sardine “Sardinella gibbosa" and cuttlefish “Sepia spp." respectively. The bacterial isolates in the examined samples were identified as Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio furnissii, Vibrio fluvialis, Vibrio mimicus and Vibrio metschnikovii. The relationship between the number of the isolated strains and Kanagawa positive phenomena in the examined samples were discussed.