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Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis for identification of red meat animal species

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Last updated: 23 Jan 2023

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Abstract

Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS- PAGE) analysis was used to identify red meat of various animal species. The protein profiles of whole muscle samples of different animal species after Coomassie- stained 12% gel showed different bands in different species. In all species (cattle, camel, horse, sheep, pig and dog), the molecular weight of the protein bands was ranged from 14.300 to 66.000 kDa, except camel, one prominent distinct band appeared above 66.000 kDa. In horse, the band pattern showing control distinct band at 34.7000. In sheep, pig and dog there were slight differences in their bands pattern which characteristic for each species. In conclusion, species speciation of meat can be done by SDS- PAGE and it is accurate, easy and comparatively rapid technique than many other methods which used for detection of adulteration of meat and meat product.  

DOI

10.21608/avmj.2007.177989

Keywords

Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis for identification of red meat animal species

Authors

First Name

M.G.,

Last Name

Aggour

MiddleName

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Affiliation

Dep. of Biotechnology, Animal Health Research Institute, Dokki

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City

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Orcid

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First Name

Souzan,

Last Name

M.A. Abu Zied

MiddleName

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Affiliation

Dept. of Food Hygiene, Animal Health Research Institute, Dokki

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City

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Volume

53

Article Issue

115

Related Issue

24390

Issue Date

2007-10-01

Receive Date

2007-09-11

Publish Date

2007-10-01

Page Start

1

Page End

9

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_177989.html

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https://avmj.journals.ekb.eg/service?article_code=177989

Order

6

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023