In this study, SDS-PAGE analysis of major polypeptides from three strains: Salmonella typhimurium, Escherichia coli O157 H7and Staphylococcus aureus isolated from chicken meat were determined. The electrophoretic profile of the three strains expressed 11, 9 and 10 proteins bands respectively. The molecular weight of the whole cell protein also showed some difference between strains which ranged from 18.48-102.70, 18.46-63.69 and 18.95-72.42 respectively. These results demonstrated that the protein profile of the three organisms isolated from chicken meat was characteristic for each organism which helpful in their diagnostic procedures due to its accuracy than traditional methods.