A total of one hundred random samples, fifty from ice- cream and 50 from yoghurt were collected from Port-Said markets. Samples were examined to isolate and evaluate the prevalence rate of E. coli O157:H7 and other serotypes. E. coli was detected in ice-cream and yoghurt samples at a rate of 58 and 42 %, respectively. 9(18 %) and 5(10 %) out of 50 ice- cream and yoghurt samples, respectively were found to be contaminated with E. coli O157: H7. A total of 66 E. coli isolates recovered from positive samples were identified to serogroups, O112(40.9%), O119(31.8%) O111(21.2%), O26(3 %) and O55(1.5%). The majority of E. coli serotypes recovered from the examined samples showed hemolytic activity. The public health significance of the isolated serogroups and consumer's safety were discussed.