The bacteriological study was carried out to evaluate the degree of contamination in indigenous cattle carcasses in slaughterhouse, in KhartoumState, during April 2009 - June 2009. For the total viable counts (TVCs), 768 swab samples were collected from 32 carcasses. Sterilization by hot tap water (82 ^ﮦC) to worker's hands and their knives was applied in all slaughtering operations. The mean total viable count (TVCs) after skinning, evisceration and washing operations at brisket site were 1.4 ± 0.12, 1.5 ± 0.09 and 1.5± 0.06 log₁₀ CFU (colony forming unit) /cm²_,in the shoulder site were, 1.3 ± 0.09, 1.2 ± 0.11 and 1.1 ± 0.05 log₁₀ CFU/cm², in the neck site were 1.7 ± 0.05, 1.4 ± 0.06 and 1.6 ± 0.06 log₁₀ CFU/cm² whereas in the rump site the TVCs in these operations were 1.4 ± 0.06, 1.1 ± 0.07, and 1.2 ± 0.10 log₁₀ CFU/cm^2., respectively with statistically significant difference (P<0.05). Also there were reduction in TVCs of worker's hands and their knives with statistically significant difference (P<0.05).