The objective of this study is, serological investigation of Epizootic Equine Abortion (EEA) or Equine Rhinopneumonitis (ER) Equine HerpesvirusMyeloencephalopathy(EHM) that know worldwide Equid herpesvirus (EHV type 1 and 4) in Syria. Equid herpesvirus 1 and 4 are widespread in the domestic horses population worldwide. EHV1 is a major cause of abortion, respiratory and neurologicaldisorders in horses. EHV4 is responsible of respiratory disease.In this study, Antibodies of EHV1 and 4 were investigated serologically in horses in Syria. A total of 203 unvaccinated horses in five regions in Syria were sampled and tested using indirect ELISA. EHV1-specific antibodies were found to be in fifth regions as 7(3.5%). EHV4- specific antibodies were found to be in fifth regions as 195(96%). This is the first serological investigation for EHV1 and EHV4 in Syria.