A total of 150 random samples of evaporated; condensed and dried milk (50 samples each) were collected from dairy shops and supermarkets in Assiut city for the presence of anaerobes. By using plate method the percentages were 40, 40 and 98 % with an average count of 1.5 × 10 ²; 4 × 10 ³ and 8.5 × 10 ² /g; respectively. Cl.species as Cl.ramosum, Cl.perfringens, Cl.difficile Cl.innoculum, Cl.septicum, Cl.novyi type A, Cl.cadaveris and Cl.subterminale were identified in varies percentages from the evaluated samples. In addition, Cl. perfringens was recorded in a percentage of 16 % with an average count of 1.5 × 10²/g of dried milk using MPN technique. Fortunately, Cl.botulinum failed to be detected in all examined samples. Moreover,8 and 2 Cl.perfringens isolated, respectively from dried and evaporated milk were negative for α-Toxin produced by Cl.perfringens by using Enzyme linked immunosorbent assay (ELISA). Public health as well as economical significance of detected microorganisms in the concentrated and dried milk were discussed.