A total of 125 random samples from raw milk, locally manufactured kareish cheese, hard cheese (Rass cheese), plain yoghurt and flavored yoghurt (25 of each) collected from dairy shops and super markets in Beni Suef city were subjected to mycological examination. The yeasts were detected in milk, Rass cheese and locally manufactured kareish samples with a mean value of 6.22Χ10² ± 3.62Χ10², 14±7.21 and 3.23Χ10⁶ ± 2.017Χ10⁶ respectively, while plain yoghurt and flavored yoghurt were free from any yeasts (<10). The most predominant isolated spp. were Candida pseudotropicalis, Rhodotorula species, Candida species, Torulopsis (candida) glabrata and Cryptococcus neoformans. The mean value of molds was 2.23Χ10²±9.3Χ10¹, 4.8±1.54, 1.068Χ10⁶±7.20Χ10⁵, and 2.4±0.871 in the examined milk, Rass, locally manufactured kareish cheese and flavored yoghurt samples respectively, while molds couldn^'t be detected in the examined plain yoghurt samples. The most predominant isolated spp. Were Mucorspp, Asperigellus flavus, Asperigellus niger, Asperigellus fumigatus, Absidia spp., Penicillium spp., Geotrachum candidum, Cladosporium spp., Phoma spp. and Fusarium spp < /em>. All the examined Rass cheese samples were free from aflatoxin M1 calibrated by fluorometer. The economic and public health significance of the fungi and mycotoxins as well as the sanitary precautions were mentioned.