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194118

PRODUCTION OF EXTRACELLULAR EMULSIFIERS FROM YEASTS FOR USING IN DAIRY AND FOOD PRODUCTS

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Last updated: 24 Dec 2024

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Abstract

The main objective of this study was to test the ability of a number of yeast strains for production extracellular bioemulsifiers for potential use in food. An emulsifying agent was extracted from each strain of yeast tested, including 9 species of genera other than Candida utilis. From the 11 products tested, eight had emulsifying properties that were better than those of commonly used as food emulsifiers' mono-glyceride (Glycerol monoleate). Candida utilis EMCC 120 was selected for further study due to the excellent emulsification properties of its extracellular product and the food grade status of the yeast strain. Extracellular bioemulsifiers extracted emulsifier was purified by ultrafiltration. The purified product was evaluated for chemical and physical stability to establish its potential use as a natural emulsifier in processed foods. The yield of purified bioemulsifier was 3.2 g l–1 of the original dry cell weight for Candida utilis EMCC 120, and 0.85 g l–1 for Saccharomyces cerevisiae EMCC 69. Carbohydrate content of the purified bioemulsifier was 76%. Protein content was 23.75 % for Candida utilis EMCC 120. Butter oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 5% sodium chloride or up to 15% sucrose in the aqueous phase. In the presence of a low concentration of various solutes, emulsions were stable to three cycles of freezing and thawing.

DOI

10.21608/ejar.2009.194118

Keywords

yeast, emulsifier, emulsification activity, Emulsion stability, Dairy product

Authors

First Name

ABEER E. A.

Last Name

AMER

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Affiliation

Dairy Microbiology Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

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First Name

SOAD A.

Last Name

SHERIF

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Affiliation

Dairy Microbiology Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

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Orcid

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First Name

IBRAHEM M.

Last Name

ROUSHDY

MiddleName

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Affiliation

Food Science Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

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First Name

MOHAMED N.

Last Name

EL- MAGDOUB

MiddleName

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Affiliation

Food Science Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

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Volume

87

Article Issue

2

Related Issue

27534

Issue Date

2009-07-01

Receive Date

2008-12-30

Publish Date

2009-07-01

Page Start

559

Page End

568

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_194118.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=194118

Order

19

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

PRODUCTION OF EXTRACELLULAR EMULSIFIERS FROM YEASTS FOR USING IN DAIRY AND FOOD PRODUCTS

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Article

Created At

22 Jan 2023