Antibiotic concentrations in broiler meat are of exceptional health difficulty to human due to its dangers on the purchaser health. This learn about supposed to display the antibiotic residues in 60 frozen broiler samples accumulated from unique hen stores and retail markets in Ismailia Governorate by the use of High-Performance Liquid Chromatography. The outcomes confirmed that 71.7% of the examined samples had been containing at least one antibiotic residue whilst 28.3% had been free from any antibiotic residue. The incidence of tetracycline and sulfonamide in the evaluated samples were 38(88.4%) and 28(65.1%) respectively. The imply awareness values of chlorotetracycline (CTC), doxycycline (DOC), oxytetracycline (OTC) and tetracycline (TTC) in chicken samples were 120.1±30, 156.12±40.86, 115.09 ±10.68 and 139.26±32.21 µg/Kg respectively. While for Sulfonamides they had been 64.16±12.41µg/kg; 75.54±15.64µg/kg; 60.75±12.34 µg/kg and 79.34±24.6 µg/kg for Sulphadiazine, Sulfadimidine, Sulfamethazinee and Sulfaquioxaline residues respectively. Based on the national and global regulations, the received consequences published that the awareness of the detected antibiotics had been exceeding the recommended most residue limit in most broilers is extraordinary hazard on the human fitness if this antibiotic overuse continues.