This study was carried out to evaluate green barley leaves (Giza 117) as a rich source of Beta carotene through feeding study to compare the bioavailability of barley leaves [Ll-carotene with synthetic B-carotene. Also in the present study green barley leaves juice was used for enrichment barely drink and guava juice with B~ carotene. The data showed that using the barley leaves diet as a source for [t- carotene had the advantage of adding extra nutrients to experimental rats comparing with other diets. These nutrients helped the rats to be healthier, and grow more rapidly. Bioavailability of barley leaves B-carotene was higher than that of synthetic B- carotene. Levels of Vitamin A were higher in plasma and liver of rats fed on barley leaves diet than that of synthetic B-carotene diet. Using barley leaves juice for fortification showed a clear effect in increasing B-carotene levels in barley drink and guava juice.