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90864

Incidence and Characterization of Bacillus Cereus in Some Meat Products Using P.C.R

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Meat hygiene

Abstract

A B S T R A C T
Bacillus cereus is widespread in nature and foods. Several members of this group are recognized as causing food spoilage and/or health issues. This study was designed to determine the prevalence and genetic diversity of B. cereus strains isolated from 120 samples of meat products represented by beef burger, sausage, luncheon and rice kofta (30 of each) which were collected from different shops and supermarkets in Gharbia governorate. Samples were examined for the presence of B. cereus group after selective plating on MYP agar and enumeration of each sample. The high incidence of B. cereus was recorded in rice kofta samples (56.7%) followed by sausage (46.7%), beef burger (43.3%) and luncheon(26.7%). With count 4.39×104± 0.58×104, 1.12×104± 0.25×104, 8.06×103± 1.69×103 and 3.73×103± 0.51×103 respectively .Further biochemical tests were carried out for identification before being subjected to PCR for diarrheal gene (HBLA gene) which has been found in 37.5%of tested isolates.

DOI

10.21608/bvmj.2019.18074.1114

Keywords

KEY WORDS: Bacillus cereus, meat products, PCR

Authors

First Name

Fatma

Last Name

Ahmed

MiddleName

-

Affiliation

Meat hygiene ,Veterinary medicine ,Benha University

Email

dr.fatma_hussien2014@yahoo.com

City

mahala

Orcid

-

First Name

Mohamed

Last Name

Hassan

MiddleName

Ahmed

Affiliation

Food Hygiene Department, Faculty of Veterinary Medicine, Banha university

Email

mohamedhsn40@yahoo.com

City

Tanta

Orcid

-

First Name

Reham

Last Name

Amin

MiddleName

-

Affiliation

Meat Hygiene Department, Faculty of Veterinary Medicine, Benha University

Email

rehamnour2007@yahoo.com

City

Benha

Orcid

-

First Name

Nesreen

Last Name

Eleiwa

MiddleName

-

Affiliation

chief researcher of food hygiene, Animal Health Research Institute, Dokki, Giza, Egypt.

Email

nesreenwow@gmail.com

City

-

Orcid

-

Volume

37

Article Issue

1

Related Issue

8030

Issue Date

2019-09-01

Receive Date

2019-10-11

Publish Date

2019-09-01

Page Start

86

Page End

90

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_90864.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=90864

Order

18

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Incidence and Characterization of Bacillus Cereus in Some Meat Products Using P.C.R

Details

Type

Article

Created At

22 Jan 2023