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88806

Molecular detection of enterotoxigenic Staphylococcus aureus in ready to eat beef products

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Meat hygiene

Abstract

Ready to eat (RTE) meat products are considered a rapid way to animal protein intake which may be preferred by consumers due to its palatability and quality; unfortunately, cooked meat products may be loaded by many foodborne pathogens especially Staphylococcus aureus (S. aureus) due to post cooking cross-contamination through mishandling and\or getting in touch with raw materials; so, this study aimed to investigate the incidence of S. aureus in some RTE meat products followed by molecular detection of enterotoxigenic S. aureus isolates and its enterotoxin genes in 120 RTE meat products represented by luncheon, burger, shawerma and kofta (30 of each) which were collected from different restaurants and street vendors in Benha city, Qalubiya governorate, Egypt. Results revealed that kofta samples were the most contaminated samples with mean values 3.42x103, and 5.2x10 CFU\g for total staphylococci and S. aureus counts, respectively; followed by burger, shawerma and luncheon samples. Multiplex PCR detection of S. aureus enterotoxins (SE) genes revealed detection of different isolates carrying staphylococcal enterotoxin type A, type B, mixed strain carrying both staphylococcal enterotoxins type A and type D was detected. So, RTE meat products of low hygienic measures may be risky to consumer's health.

DOI

10.21608/bvmj.2019.15640.1062

Keywords

meat products, Staphylococcus aureus, enterotoxin, Multiplex PCR, Benha City

Authors

First Name

Marwa

Last Name

Saif

MiddleName

-

Affiliation

faculty of Veterinary Medicine. Benha University.

Email

marwasaif09@gmail.com

City

-

Orcid

-

First Name

Saad

Last Name

Saad

MiddleName

-

Affiliation

Food control Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt

Email

saadms44@yahoo.com

City

-

Orcid

-

First Name

Fahim

Last Name

Shaltout

MiddleName

-

Affiliation

Food control Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt

Email

fahim.shaltout@fvtm.bu.edu.eg

City

Toukh

Orcid

https://orcid.org/my

First Name

Fatin

Last Name

Hassanin

MiddleName

Said

Affiliation

Food control Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt

Email

fatinsaidhassanin@yahoo.com

City

-

Orcid

-

First Name

Marionette

Last Name

Zaghloul

MiddleName

-

Affiliation

Food Hygiene Dept., Animal Health Research Institute, Benha branch

Email

marionettezaghloul@yahoo.com

City

-

Orcid

-

Volume

37

Article Issue

1

Related Issue

8030

Issue Date

2019-09-01

Receive Date

2019-08-04

Publish Date

2019-09-01

Page Start

7

Page End

11

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_88806.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=88806

Order

2

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Molecular detection of enterotoxigenic Staphylococcus aureus in ready to eat beef products

Details

Type

Article

Created At

22 Jan 2023