A total of 100 random samples were collected from surfaces of cattle carcasses in EL-Shouhada and Birket El –Sabaa Abattoirs in Menofyia governorate (50 of each).The results showed that the mean E. coli counts were 5.44×102 and 7.99×102 cfu/cm2 in EL- Shouhada and Birket EL-Sabaa abattoirs, respectively. Also, the incidence of E .coli isolated from cattle carcass surfaces in EL-Shouhada and Birket El-Sabaa abattoirs were 26% and 28%, respectively. The isolated serotypes of E. coli were EHEC as O111 : H2 (8%) and O26 : H11 (2%), EPEC as O15 : H4 (2%), O55 : H7 (2%), O114 : H4 (4%) and  O146 : H21(2%), ETEC as O128 : H2  (2%) and O125: H21 (2%) and EIEC as O124 (2%) in EL- Shouhada abattoirs, while in Birket EL-Sabaa abattoir were EHEC as O111 : H2 (6%) , O26 : H11 (8%) and O103 : H4 (2%), EPEC as O55 : H7 (2%) and O91 : H21(2%) ETEC as O128: H2 (6%) and EIEC as O124 (2%).  Findings of multiplex PCR showed that eaeA (intimin) gene was detected in (O91:H21 and O114:H4), hlyA (haemolysin) gene was detected in (O125:H21), stx1 gene was not detected in the all isolated E. coli serogroups, but stx2 gene was detected in (O26:H11 and O114:H4).