A total of 100 random samples of fresh and marine fishes represented by Tilapia niloticus, Mugil cephalus, Sardine and Barboni (25 0f each) were collected from the different fish markets located in Gharbia governorate. All collected samples were subjected to bacteriological examination for isolation and identification of Enterobacteriaceae. The results revealed that the total Enterobacteriaceae  counts in the examined samples of fresh and marine fish were varied from 1.4×103 to 5.7×104 with a mean value ( 1.9×104 )cfu/g  for Oreochromis niloticus ,7.0×102 to 2.40 ×104 with a mean value (9.06×103 )cfu/g  for  Mugil cephalus , 4.0×102 to 8.9×103 with a mean value, 4.25×103cfu/g  for Sardine , 1.0×102 to 3.30 ×103 with a mean value ( 1.18×103 )cfu/g  for Barboni. Also, Salmonella species were recovered from 24 %, 20 %, 8% and 8 % of the examined samples of O. niloticus, Mugil cephalus, Sardine and Barboni, respectively. Enterobacteriaceae count, Coliform count and the isolated Salmonella organisms as well as the public health significance were discussed and the prophylactic measures to reduce contamination in fish were recommended The work was aimed to study occurrence of Enterobacteriaceae in fish sold in different fish markets located in Gharbia governorate.