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47885

The Molecular and Cytogenetic Characterization of Benha Line Chickens

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Last updated: 04 Jan 2025

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Animal Production

Abstract

Benha line chickens (Line B) were originated by crossing the Egyptian native Golden Montazah cocks with the White Leghorn chickens hens. The main aim of this study was to estimate the genetic characterization of Benha Line Chickens compared to Golden Montazah and White Leghorn chickens as parents. In this study, the genomic DNA of B line, Golden Montazah and White Leghorn chickens were extracted to carry out Polymerase Chain Reaction (PCR) using five specific microsatellite markers (MCW0330, MCW0183, MCW0081, MCW0014 and ADL0278). These markers are specific for egg number, egg shell strength, clutch size, breast muscle weight (BMWT), body weight, egg production rate, heat stress and growth. Further, MCW0330 and MCW0081 primers are specific for resistance of Salmonella and Marek's (MD) diseases. Our results confirm that these specific microsatellite markers are specific for the Benha line chickens except the ADL0278 not specific for Benha line chickens. Further, 26 distinct alleles were detected at the five microsatellite markers. Amongst 26 distinct alleles, Benha Line was carried the largest number of specific alleles at five (20.8%), followed by Golden Montazah with two specific alleles (10.5%). In addition to, the polymorphic information content (PIC) and expected heterozygosity (He) for the three chicken populations were calculated. Benha Line chickens were the highest values of PIC and He per locus which were observed at MCW0183, MCW0330 and ADL0278, respectively, and the corresponding lowest were at MCW0014 and MCW0081, respectively. Moreover, chromosomal analysis was carried out and our results confirm that Benha Line chickens have a diploid number of 78 (2n = 78) chromosomes and the majority are microchromosomes. 

DOI

10.21608/bvmj.2018.47885

Keywords

Benha line chicken, weight, Microsatellite markers, resistance, chromosomal analysis

Authors

First Name

M. M.

Last Name

Iraqi

MiddleName

-

Affiliation

1Department of Animal Production, Faculty of Agriculture, Benha University, Egypt

Email

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City

-

Orcid

-

First Name

M.M

Last Name

Bekhit

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

M.H

Last Name

Refaat

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

F

Last Name

Soudy

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

M. M-El

Last Name

Attrouny

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture, Benha University, Egypt.

Email

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City

-

Orcid

-

First Name

A

Last Name

Mansour

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

M.M.

Last Name

Abdelrhamn

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

O.F

Last Name

Abdelkreim

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

A.A

Last Name

Aboshady

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

O.M.

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Egypt. Moshtohor Research Park, Molecular Biology Lab., Benha University, Egypt.

Email

-

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

6374

Issue Date

2018-08-01

Receive Date

2019-09-12

Publish Date

2018-08-01

Page Start

386

Page End

398

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 34_Issue 3_Pages 386-398.pdf

PDF . 697.6kB

Link

https://bvmj.journals.ekb.eg/article_47885.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=47885

Order

32

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

The Molecular and Cytogenetic Characterization of Benha Line Chickens

Details

Type

Article

Created At

22 Jan 2023