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Conventional and modern identification techniques for identification of Salmonellae isolated from some meat products

Article

Last updated: 04 Jan 2025

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Abstract

This study was conducted to determine the prevalence of Salmonella pathogens in minced meat, kofta,
beef burger and sausage (25 samples of each), weight of each sample10gm were collected from different
shops and supermarkets in Cairo governorate. The obtained results indicated that the incidence of
Salmonellae isolated from examined samples were (2)8%, (2)8%, 0% and (1)4% of minced meat, kofta,
beef burger and sausage respectively. The serotypes of 5 positive Salmonella isolates in the examined
meat product samples were Salmonella Typhimurium, Salmonella Muenster and Salmonella Enteritidis.
Modern identification techniques as Enzyme-linked immunosorbent assay (ELISA), Latex
Agglutination and Polymerase Chain Reaction (PCR). These techniques are considered powerful, rapid
procedure with both sensitivity and specificity for quick detection and identification of specific
pathogenic bacteria from different sources. The results obtained by applying PCR on 5 isolated
Salmonella strains show presence of common Salmonella gene in isolated strains, the identification of
isolated Salmonella strains show positive results by ELISA and latex agglutination technique only
confirm Salmonella Typhimurium and Salmonella Enteritidis. Since presence of these food poisoning
microorganisms as Salmonella constitute a potential risk to public health, these findings underscore the
need to control them and to limit bacterial multiplication in meat products.

DOI

10.21608/bvmj.2015.31732

Keywords

Salmonellae, meat products, ELISA, PCR

Authors

First Name

Hemmat

Last Name

Ibrahim

MiddleName

M.

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University

Email

drhemmat01@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Hassan

MiddleName

A.

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University

Email

mohamed.hassan@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Reham

Last Name

Amin

MiddleName

A.

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University

Email

rehamnour2007@yahoo.com

City

-

Orcid

-

First Name

Nesreen

Last Name

Eleiwa

MiddleName

Z.

Affiliation

Meat hygiene, animal health research institute Tanta Branch

Email

-

City

-

Orcid

-

First Name

Samaa

Last Name

Nadim

MiddleName

S.

Affiliation

Meat hygiene, animal health research institute Tanta Branch

Email

-

City

-

Orcid

-

Volume

29

Article Issue

2

Related Issue

5360

Issue Date

2015-12-01

Receive Date

2019-05-14

Publish Date

2015-12-01

Page Start

262

Page End

267

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_31732.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=31732

Order

32

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Conventional and modern identification techniques for identification of Salmonellae isolated from some meat products

Details

Type

Article

Created At

22 Jan 2023