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Prevalence and molecular characterization of Pseudomonas species in frozen imported meat

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Last updated: 24 Dec 2024

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Abstract

A total of 90 random samples of Frozen American, Brazilian and Indian meat samples (30 of each) were collected from
different retail shops and supermarkets in EL-Menofeya Governorate at different production dates. The collected samples
were examined bacteriologically and by using PCR tecnique for detection of Pseudomonas species, especially
Ps.aeruginosa. There for , Ps. aeruginosa , Ps.alcaligenes , Ps.cepacia , Ps. fluorescence , Ps. proteolytica , Ps.
Psychrophila, Ps. putrifaciens, Ps.thermotolerans, Ps. versicularis , Ps. fragi,Ps.Putida Ps.orientalis and Ps.stutzeri were
isolated from 2(6.67%), 5 (16.67%), 1 (3.33%), 14 (46.67%), 8 (26.67%), 3(10%), 10 (33.33%),6(20%), 1 (3.33%) and
zero for Ps.fragi,Ps.Putida,Ps.orientalis and Ps.stutzeri for frozen American meat samples ,3 (10%) , 8 (26.67%) , 1
(3.33%) , 19 (63.33%),5(16.67%), 7 (23.33%), 13(43.33%), 9 ( 30%) 3(10%) , 2(6.67%) , 1(6.67%) and zero for
Ps.orientalis one Ps.stutzeri , for frozen Brazilian meat samples, 6(20%),9(30%),4 (13.33%),25(83.33%),6 (20%),
11(36.67),19(63.33%) , 13(43.33%), 4(13.33%), 3(10%), 3(10%), 1(3.33%) and 2(6.67%) for frozen Indian meat
samples, respectively. Regarding to Ps.aeruginosa the total number and percentage of ps.aeruginosa were 2(6.67%) ,
3(10%) and 6(20%) for American , Brazilian and Indian frozen meat, respectively with total result of (12.22%). By using
PCR technique, the all examined samples by conventional method showed positive result by both of PCR and
conventional method to Pseudomonas species. On the other hand of Ps. aeruginosa was detected in one sample of Indian
meat by conventional method while it showed negative result by PCR technique

DOI

10.21608/bvmj.2016.31301

Keywords

Pseudomonas, frozen meat, Molecular characterization, PCR, PA- GS, PA-SS

Authors

First Name

Hemmat

Last Name

Ibrahim

MiddleName

M.

Affiliation

Food Control Dept., Fac. Vet. Med., Benha University

Email

drhemmat01@yahoo.com

City

-

Orcid

-

First Name

Hassan

Last Name

A.

MiddleName

M.

Affiliation

Food Control Dept., Fac. Vet. Med., Benha University

Email

-

City

-

Orcid

-

First Name

Nahla

Last Name

bou El-Roos

MiddleName

A.

Affiliation

Animal Health Research Institute, Shbin Elkoom

Email

-

City

-

Orcid

-

First Name

Mohga

Last Name

Abd Elsalam

MiddleName

-

Affiliation

Animal Health Research Institute, Shbin Elkoom

Email

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City

-

Orcid

-

Volume

31

Article Issue

2

Related Issue

5342

Issue Date

2016-12-01

Receive Date

2019-05-08

Publish Date

2016-12-01

Page Start

220

Page End

224

Print ISSN

1110-6581

Online ISSN

2974-4806

Link

https://bvmj.journals.ekb.eg/article_31301.html

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https://bvmj.journals.ekb.eg/service?article_code=31301

Order

31

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Prevalence and molecular characterization of Pseudomonas species in frozen imported meat

Details

Type

Article

Created At

22 Jan 2023