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31258

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

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Last updated: 04 Jan 2025

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Abstract

Shiga toxins were widely spread in the meat especially in minced meat. These toxins have an important significance to
human health because it is a major cause of food poisoning. About 150 meat samples purchased from a number of
supermarkets and butcher shops in Luxor city were examined for presence of E. coli (50 raw meat samples - 50 minced
meat samples - 50 sample of chicken meat). 62 samples were positive for E.coli spp. 26 isolates were confirmed
serologically using O &H specific antisera as E.coli. Incidence of E. coli was in chicken meat 6/50 (12%) and raw meat
11/50 (22%) and minced meat 9/50 (18%). 26 E. coli isolates tested serology using special antisera (O & H) recognize
that there are 12 genetic groups They are O26: H11, O114: H21, O119: H4, O2: H6, O125: H21 in chicken meat. O111:
H2, O55: H7, O125: H21, O128: H2, O26: H11, O124 in raw meat. In minced meat O128: H2, O119: H4, O44: H18,
O26: H11, O111: H2, O78, O55: H7. E.coli O111,O26 and O119 the most prevalent serotype. Polymerase chain reaction
was used to detect virulence genes in isolated strains stx1, stx2, eaeA

DOI

10.21608/bvmj.2016.31258

Keywords

E. coli, stx1, stx2, eaeA

Authors

First Name

Jehan

Last Name

Daoud

MiddleName

R.

Affiliation

Faculty of Vet Medicine, South Valley Uni.

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Orcid

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First Name

Karmi

Last Name

Mohamed

MiddleName

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Affiliation

Faculty of Vet Medicine, Aswan Uni.

Email

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City

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Orcid

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First Name

Soad

Last Name

Nasef

MiddleName

A.

Affiliation

Reference Lab. For Vet. Quality control on poultry production

Email

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City

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Orcid

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First Name

Reham

Last Name

Ahmed

MiddleName

Y.

Affiliation

Reference Lab. For Vet. Quality control on poultry production

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-

City

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Volume

31

Article Issue

2

Related Issue

5342

Issue Date

2016-12-01

Receive Date

2019-05-07

Publish Date

2016-12-01

Page Start

40

Page End

44

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_31258.html

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https://bvmj.journals.ekb.eg/service?article_code=31258

Order

6

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

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Article

Created At

22 Jan 2023