There is a demand to find alternative medicine to treat the salivary glands, as any injury to the salivary glands generates an abscess. The study aimed to evaluate the antioxidant impact of walnut oil on lowering the state of oxidative stress caused by hydrogen peroxide. Thirty rats were divided into three groups of ten rats each. G1, control received a daily dose of 0.2 g/kg of walnut oil. G2 was given 0.5% H2O2 drinking water every day. G3 was given 0.5% H2O2 drinking water every day. Walnut oil at a dose of 0.2 g/kg was given orally once a day. The treatment periods were 3, 7, and 14 days. The results revealed that the level of total antioxidant capacity TAC increased significantly with a drop in MDA in the walnut oil group over time, whereas the level of TAC decreased dramatically with an increase in MDA in the H2O2 group. This finding was reversed when walnut oil was added to the administration, especially after 14 days of treatment. The histopathological changes revealed the presence of an improving effect of walnut oil against the oxidizing effect of H2O2, as the pathological effects associated with the use of H2O2 with walnut oil were reduced when compared to the H2O2 group alone, which showed necrosis of the striated ducts, and degeneration of the convoluted tubules and mucous acinar cells. Vascularisation as well as increased fibrous tissue. We concluded that walnut oil has antioxidant properties that can counteract the oxidative effects of H2O2 in rat salivary glands.