Vitamin C, a water-soluble antioxidant, is important in almost every phase of wound healing. However, oral routes provide some obstacles to delivering vitamin C to the skin, such as absorption limits and issues in achieving adequate bioavailability. Consequently, the current study aimed to evaluate the effectiveness of various dosages of topically applied vitamin C solution on intentional skin wound healing. 18 male albino rabbits were divided into three groups according to the time of euthanasia.  Each rabbit had four full-thickness excisional skin wounds on its dorsum; one was left untreated, the second had ultrasound gel applied to it, the third had 5% topical vitamin C, and the fourth had 10% vitamin C (both covered by ultrasound gel). The animals were euthanized after 24 hours, 3 days and 7 days and MMP-9 was used for immunohistochemical analysis. The group that received 10% vitamin C had significant increases in MMP-9 expression on the first and fifth days, while the control group and the ultrasonic gel group both displayed a moderate increase. According to the study, using vitamin C topically accelerates the healing of skin wounds; higher concentrations (10%) have more beneficial effects.