In this study, tapeworms of the genus Raillietina were diagnosed in Brahamas chickens in Mosul city, which suffered from emaciation and weakness, as some tapeworms were excreted in the chicken's feces. The mature segments of these worms stained with carmine appeared red in color and contained one set of reproductive organs and a regular unilateral genital opening. Both the ovary and the vitelline glands formed a ring-like shape located in the middle of the body segments. Gravid segments were distinguished by containing egg capsules that contain a number of eggs. The diagnosis of these worms was confirmed molecularly using the polymerase chain reaction technique based on 18srRNA and ITS genes, as well as with a molecular weight of 500 bp and 800 bp. Genetic sequencing results showed the diagnosis of two new isolates: Raillietina tunetensis SNEM1 gene for 18S rRNA of the partial sequence (LC764447.1) and Raillietina sonini SNEM2 gene for 18S rRNA of the partial sequence (LC764448.1). A phylogenetic tree recorded isolates based on a program Mega 11, as the ratio of the genetic proximity of the isolates to the Raillietina echinobothrida China isolate was 1.439. It was very far from the strain Raillietina tunetensis by 0.214.