422929

OCCURRENCE AND MOLECULAR DETECTION OF CAMPYLOBACTER JEJUNI AND COLI IN TABLE EGGS AND STOOL OF DIARRHEIC PATIENTS

Article

Last updated: 27 Apr 2025

Subjects

-

Tags

Zoonotic diseases and public health

Abstract

Inappropriate handling of eggs can still result in infection from bacteria such as Campylobacter, which mostly contaminates the surface of the eggshell, so this study focused on the molecular detection of Campylobacter jejuni and Campylobacter coli in table eggs and stool of diarrheic patients. The conventional PCR was carried out on 40 egg samples, 20 from each farm and baladi types equally, and 20 stool samples from diarrheic patients.  It was found that Campylobacter spp. infection was prevalent in farm and baladi eggshell samples, and positive C. jejuni DNA was detected in 45% and 15%, respectively. In comparison, 50% and 40% of farm and baladi egg shell samples were positive C. coli. However, neither C. jejuni nor C. coli DNA was detected in the egg contents of either farm or baladi eggs. The prevalence of Campylobacter spp. infection was 70% and 50% in farm and baladi eggshell samples, respectively. C. coli was detected in 50% of diarrheic patients' stool, while C. jejuni wasn't detected. The prevalence of C. coli infection was higher in females than males. The 3Y-5Y age group had the highest prevalence (75%) and the lowest (40%) was in the more than 5Y group. The prevalence of C. coli infection in diarrheic patients was highest in patients with vomiting, followed by dehydration, abdominal pain, and fever. The study recommended good hygienic practices inside poultry farms and farmer's houses and increasing awareness of poultry farm workers and farmers from infection.

DOI

10.21608/avmj.2025.354429.1559

Keywords

Eggs, stool, C. jejuni, C. coli, PCR

Authors

First Name

MARWA

Last Name

ABD EL KADER

MiddleName

G.

Affiliation

Department of Food Hygiene, Safety and Technology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt.

Email

marwagamalvetmilkhygiene@gmail.com

City

Assiut

Orcid

0009-0002-6312-5006

First Name

REEM

Last Name

ALSAADAWY

MiddleName

M.

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt.

Email

reem.barbary@vet.au.edu.eg

City

-

Orcid

-

First Name

ASMAA

Last Name

RAYAN

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut Egypt.

Email

asmaa-adel@aun.edu.eg

City

-

Orcid

-

Volume

71

Article Issue

185

Related Issue

55112

Issue Date

2025-04-01

Receive Date

2025-01-21

Publish Date

2025-04-01

Page Start

276

Page End

288

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_422929.html

Detail API

http://journals.ekb.eg?_action=service&article_code=422929

Order

20

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

OCCURRENCE AND MOLECULAR DETECTION OF CAMPYLOBACTER JEJUNI AND COLI IN TABLE EGGS AND STOOL OF DIARRHEIC PATIENTS

Details

Type

Article

Created At

27 Apr 2025