The present research was conducted to distinguish Babesia spp in sheep in different locations in the city of Mosul - Iraq, using conventional molecular methods. One hundred and eighty blood samples were collected from local sheep of different age groups and of both sexes for the period from 1/12/2023 to 1/5/2024. The blood samples were examined using PCR technique, genetic sequencing, and a phylogenetic tree based on the 18srRNA gene. Our results showed that several positive samples infected with Babesia parasite reached 132 samples, at a rate of 73.3%, with a reaction result of 300 base pairs. In this research, three new isolates of the Babesia ovis (LC799506.1, LC799507.1, and LC799508.1) and three new isolates of the Babesia motasi (LC799509.1, LC799510.1, and LC799511.1) were recorded in sheep in Mosul city. The Babesia ovis isolates fall within the scope of the genetic sequence and phylogenetic tree within the same clade and that these isolates were closely related to both the Turkish (HM241889.1) and German (AY260178.1) isolates. As for the three isolates of Babesia motasi, there was closeness between the two isolates LC799510.1 and LC799511.1 Also, these two isolates were close to the Chinese isolates (MH899762.1 and (MF361088.1) and the British isolate (KU2345270.1). While the isolate LC799509.1 was close to the Iraqi isolate recorded in Diwaniyah KP998110.1), as well as to the isolate recorded in Pakistan (KY765559.1). In conclusion, Veterinarians and researchers should consider that Babesia ovis and Babesia motasi were diagnosed in sheep for the first time in Mosul city \ Iraq.