There is scientific evidence that nanotechnology might increase the stability, delivery, and cellular absorption of medications. Therefore, this study was designed to investigate Yucca shidigera (YS) extract nanoemulsion efficacy in the prevention and treatment of coccidiosis in broilers. YS extract 5% was formulated as nanoemulsion (YSN) and then characterized by using zeta sizer and zeta potential. Six groups of 35 chicks/group, one-day-old each, were categorized as negative control, positive control, 2 treatment groups of YS and YSN, and 2 prophylaxis groups of YS and YSN. The treatment groups were administered YS and YSN at day 6 post-infection, while the prophylaxis groups received YS and YSN 5 days before infection. The YS and YSN doses were 100 mg/L in water. All groups except the control negative were orally infected with about 25 × 10³ Eimeria tenella sporulated oocysts per chick at day 23 of the chicks' age. Both treatment and prophylaxis groups revealed a significant decrease in oocyst count, rapid recovery from the disease, and better body weight gain than the infected untreated group. The blood chemistry and hemograms in the treatment and prophylaxis groups showed no significant difference from the control infected untreated group. The pathological pictures showed a lower number of parasitic stages in the cecal tissue in the treated groups, compared to the control-infected untreated chicks. In conclusion, YS and YSN mitigate the pathogenicity of E. tenella through a reduction in the oocyst count and improve body weight gain. The present study reported no significant difference between YS and its nanoemulsion form.