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413271

Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress

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Last updated: 29 Mar 2025

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Abstract

Targeted mRNAs cleavage is directed by a class of tiny RNAs known as microRNAs (miRNAs). Different plant processes are regulated by miRNAs, including senescence, responses to environmental stressors, and development. The current study identified the senescence regulation of miRNAs. It evaluated the impact of miRNA-directed modifications in expression of target genes of Triticum aestivum cultivars in response to drought. Two cultivars of spring wheat, Sahel-1and Gemiza-10 were used in this study. The Fv/Fm ratio, indicative of the maximum quantum efficiency of photosystem II, and the Performance Index (PI), an indicator for assessing sample vitality, exhibited significantly elevated values in the drought-tolerant cultivar relative to the drought-sensitive cultivar.Furthermore, quantitative PCR analyses for the miRNAs families; miR156, miR172, miR164, miR171, miR156, and miR319 and their putative target genes at reproductive phase under drought conditions in the two genotypes were performed. The up-regulation of AP2 in the Sahel-1genotype played a crucial role in leaf wilting and delaying aging under stress of drought. The miR164 down-regulation and up-regulation of NAC1 contributed to the delay of leaf wilting and senescence.

DOI

10.21608/ejbo.2024.312534.2965

Keywords

microRNA, Drought stress, senescence, Triticum aestivum

Authors

First Name

Sandy

Last Name

Khaled

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt

Email

sandykhaled@agr.asu.edu.eg

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Abo Doma

MiddleName

H.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt

Email

aabodoma2000@gmail.com

City

cairo

Orcid

0000-0002-8939-4485

First Name

Eman

Last Name

Fahmy

MiddleName

M.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt

Email

eman_abdelhameed@agr.asu.edu.eg

City

-

Orcid

-

First Name

Fatthy

Last Name

Abdeltawab

MiddleName

M.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt

Email

fatthy38@yahoo.com

City

-

Orcid

-

First Name

Shaimaa

Last Name

Khalil

MiddleName

R.M.

Affiliation

Oil Crops Biotechnology Lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), 12619, Giza, Egypt

Email

shaimaa.rm@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdelsattar

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

Email

mteima@ageri.sci.eg

City

-

Orcid

0000-0003-0851-8309

Volume

65

Article Issue

2

Related Issue

54503

Issue Date

2025-03-01

Receive Date

2024-08-29

Publish Date

2025-03-16

Page Start

249

Page End

258

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 65_Issue 2_Pages 249-258.pdf

PDF . 1.4MB

Link

https://ejbo.journals.ekb.eg/article_413271.html

Detail API

http://journals.ekb.eg?_action=service&article_code=413271

Order

15

Type

Regular issue (Original Article)

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress

Details

Type

Article

Created At

29 Mar 2025