There has been a lot of experimental research done to identify alternative, sustainable, renewable, and ecologically friendly fuels because of the environmental pollution and lack of energy security caused by engines using conventional fuels. The purpose of this study is to develop the additives for use as a fuel mix with 45% Biodiesel as fuel, which will be used to feed the diesel engine of a single cylinder, four strokes. The engine will be tested experimentally with varying loads at fixed speed of 1500 rpm, using blends of [(5% PRETSONE additive + 50%Diesel + 45% Biodiesel fuel), (5% THERMOL-D additive + 50% diesel +45%Biodiesel fuel), (5% ECO diesel additive + 50% diesel + 45% Biodiesel fuel), (5% ABRO additive + 50% Diesel + 45%Biodiesel fuel)], respectively. The impact of varying blends of additives on engine performance and emissions has been studied and evaluated. The results of the experiment show that using 45% biodiesel and additive blends can be used in the diesel engine under the same operating conditions with increasing brake thermal efficiency by 4 % in case of THERMOL-D additive, decreasing exhaust temperature by 5% for ABRO additive than 45% Biodiesel, and decreasing brake specific fuel consumption by 4% for THERMOL-D additive. The obtained results show that Carbon monoxide CO, Carbon dioxide CO2, Soot level emissions, and NOx emissions decreased by 32%,38%,11% and 36%, respectively for THERMOL-D additive. In addition, the Oxygen O2concentration was increased by 11% for THERMOL-D additive.