Production of single-cell protein (SCP) from yeast cells is a highly promising process for providing adequate protein source for food manufacturing applications. This research designed to manufacture single-cell protein (SCP) from Trichosporon insectorum AUMC13761 that possess the ability to grow on wastewater of potato chip manufacturing. The selected yeast strain was isolated from the wastewater of potato chip manufacturing and subsequently identified using molecular techniques. Trichosporon insectorum AUMC13761 showed high biomass (24.92g dry cells/L) and protein yield (34.31g protein/100 g dry cells). Cytotoxicity SRB test was conducted, which confirmed its safety and suitability for use, whereas Trichosporon insectorum AUMC13761 biomass exhibited an IC50 value greater than 100μg/ml. Furthermore, an analysis was conducted to determine the quantitative and qualitative composition of the Trichosporon insectorum AUMC13761 biomass in terms of amino acids. Trichosporon insectorum AUMC13761 biomass contained all the essential amino acids and exhibited high content of total amino acids, reaching 11.2% (w/w) It is best to write a recommendation for the research.