Essential oil obtained from Citrus aurantifolia has been shown to have various biological activities. Hence, it has been traditionally used in the management of numerous diseases and has the prospects of being developed into valuable drugs. A total of forty-eight volatile compounds of biological interests were characterized qualitatively and quantitatively using GC/MS technique. These compounds comprise two main groups, monoterpenes (67.39%) and sesquiterpenes (25.08%). The more abundant components of C. aurantifolia essential oil were α-terpineol (9.78%), p-cymene (9.32%), β-pinene (7.68%), D-limonene (6.64%), 1-terpinen-4-ol (5.91%), (+)-α-himachalene (5.87%), citronellol (5.81%), linalool (5.57%), geraniol (4.40%), α-citral (3.92%) and α-bergamotene (3.15%).