410648

Host Range of Lytic Bacteriophages as Biocontrol Agents for Pathogenic Bacteria Causing Foodborne Illnesses in the Vannamei Shrimp (Litopenaeus vannamei)

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Last updated: 08 Feb 2025

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Abstract

Ensuring the quality of shrimp is essential to prevent exposure to Vibrio sp. bacteria. Consuming seafood contaminated with these bacteria can cause food poisoning. Vibrio parahaemolyticus is a bacterium responsible for countless cases of foodborne illness. Prolonged use of antibiotics to treat these infections may lead to antibiotic resistance. As a safer alternative, bacteriophages can be used to combat Vibrio parahaemolyticus infections. Bacteriophages are viruses that specifically target bacteria. This study aimed to isolate bacteriophages from blood clams and green mussels. Bacteriophages isolated from blood clams (Anadara granosa) and green mussels (Perna viridis) were effective in lysing the bacteria, as demonstrated by plaque formation on double-layer agar plates. The bacteriophage density from blood clams was 3.7 × 10⁴ PFU/mL, while from green mussels, it was 1.38 × 10⁷ PFU/mL. Host range tests showed that both bacteriophages were capable of lysing Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio cholerae, Vibrio harveyi, and Aeromonas hydrophila.

DOI

10.21608/ejabf.2025.410648

Keywords

Vibrio parahaemolyticus, bacteriophage, Shrimp quality, Antibiotic resistance, foodborne illness

Authors

First Name

Kinanti

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et al.

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Volume

29

Article Issue

1

Related Issue

52748

Issue Date

2025-01-01

Receive Date

2025-02-07

Publish Date

2025-01-01

Page Start

1,393

Page End

1,407

Print ISSN

1110-6131

Online ISSN

2536-9814

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https://ejabf.journals.ekb.eg/article_410648.html

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http://journals.ekb.eg?_action=service&article_code=410648

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76

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Host Range of Lytic Bacteriophages as Biocontrol Agents for Pathogenic Bacteria Causing Foodborne Illnesses in the Vannamei Shrimp (Litopenaeus vannamei)

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Article

Created At

08 Feb 2025