The study looked at the antibacterial activity of water, methanol, acetone, and ethyl acetate extracts from cape gooseberry (Physalis peruviana) against some food-borne pathogens. Plant extracts were diluted to different concentrations (50, 100, 150, and 200 mg/L) and tested against three types of bacteria namely Salmonella enterica and Escherichia coli as Gram negative bacteria and Enterococcus faecalis as a Gram positive bacteria. The obtained result observed that, all the cape gooseberry extracts under examination contained saponins, terpenes, and flavonoids, according to the chemical analysis. Additionally total flavonoids and total phenols were detected. Microbiological analysis revealed that ethyl acetate extract was more efficient than the other extracts at both 50 and 200 mg/L against the Salmonella enterica strain. These results are promising to be applied the cape gooseberry extracts as bioactive agents in the field of biotechnology.