Abstract        This study was carried out to investigate the effect of ruta graveolens extract on hepato and nephrotoxicity induced by gentamicin on experimental rats. 35 albino rats of Sprague-Dawley strain were randomly distributed after adaptation period into two main groups (7 of each), the first main group was kept on the basal diet as negative control (-ve), The rest of the animals (n = 28) were subcutaneously injected with gentamicin (100ml/ kg body weight/ 7day/i.p) for the induction of hepatotoxicity and nephrotoxicity  .After injection rats were divided into 4 subgroups. The first subgroup of rats was as a positive control group (+ve). The other subgroups were fed on the experimental diets containing different levels of ruta graveolens extract 5%, 10% and 15%, respectively. At the end of the experimental period (6 weeks) liver and kidneys functions were determined. Also, antio xidants enzymes were determined. Histopathological examinations for liver were done. The results indicated that liver enzymes ALT, AST and ALP were decreased in groups 3, 4 and 5, respectively compared with the positive control group. Uric acid and creatinine were significantly improved in all tested groups compared with the positive control one.  Moreover, antioxidant enzymes (GSH, SOD and CAT) were significantly improved in all examined groups compared with the positive control group. It could conclude that Ruta Graveolens had a potential effect against on hepato and nephrotoxicity damage therefore this study recommends increasing dietary intake of Ruta Graveolens could be beneficial for patients with hepatic and nephrotoxicity damage.