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PREVALENCE OF BACILLUS CEREUS AND DETECTION OF SOME ENTEROTOXIGENIC GENES WITH QUALITY EVALUATION IN SOME FAST-FOODS

Article

Last updated: 13 Jan 2025

Subjects

-

Tags

Meat hygiene & meat by-products

Abstract

Sixty samples of fast-food sandwiches were collected randomly from various fast-food outlets in Kafrelshiekh governorate including beef patties (Egyptian Hawawshi), beef burgers and chicken shawarma sandwiches (20, each). Bacillus cereus and quality parameters were assessed in the collected samples. The incidence rates of B. cereus were 30%, 15% and 15%, with mean values 3.02 ± 2.83, 2.01 ± 1.68 and 2.52 ± 2.08 log10 cfu/g in beef patties, beef burgers and chicken shawarma sandwiches, respectively.  PCR findings confirmed 12 isolates, which were positive for the groEl B. cereus gene. Additionally, 33.33% of sandwiches that initially tested negative for bacterial isolation carried the groEl B. cereus gene. The enterotoxin genes hbl and cytK were present in 41.66% and 91.66% of the identified B. cereus isolates, respectively. Ginger essential oil (GEO) exhibited substantial antibacterial activity against B. cereus isolate at Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), about 25% and 30%, respectively. Quality criteria including Aerobic Plate Count (APC) and chemical analysis (pH, TVBN, and TBARS) were performed on the samples. The results revealed that 30% and 10% of the examined beef patties and beef burger sandwiches, respectively, reached the unsatisfactory limit of APC stated by the Center for Food Safety (≥ 105/g). In addition, a substantial positive correlation was observed between pH, TVBN, TBARS and APC values in the examined samples. Therefore, sanitary procedures should be followed when preparing ready-to-eat (RTE) meals to reduce microbial contamination and public health hazards. Moreover, incorporating spices like ginger into RTE foods could assist in minimizing the presence of Bacillus cereus and improve food quality.

DOI

10.21608/avmj.2024.313538.1355

Keywords

Aerobic plate count (APC), Bacillus cereus enterotoxins, chemical parameters, Ginger essential oil, meat products sandwiches

Authors

First Name

WALAA

Last Name

ELKASSAS1

MiddleName

MOHAMED

Affiliation

Department of Food Hygiene, Animal Health Research Institute, Kafrelsheikh Lab, Agriculture Research Center (ARC), Egypt.

Email

w.elkasas77@yahoo.com

City

Egypt, kafrelsheikh government

Orcid

0000-0001-5077- 1223

First Name

SHEREEN

Last Name

YASSIN

MiddleName

Abdelfattah

Affiliation

Department of Food Hygiene, Animal Health Research Institute, Kafrelsheikh Lab, Agriculture Research Center (ARC), Egypt.

Email

shereen_color@yahoo.com

City

Egypt, kafrelsheikh government

Orcid

0000-0003-3732-508x

First Name

HALA,

Last Name

ABDELHADY

MiddleName

AMIN M.

Affiliation

Department of Bacteriology, Animal Health Research Institute, Kafrelsheikh Lab, Agriculture Research Center (ARC), Egypt.

Email

halaamin144@yahoo.com

City

kafrelsheikh, Egypt

Orcid

0000-0002-2178-7741

First Name

NEHAL

Last Name

NAENA

MiddleName

ALI A.

Affiliation

Department of Bacteriology, Animal Health Research Institute, Kafrelsheikh Lab, Agriculture Research Center (ARC), Egypt.

Email

nehalali978@yahoo.com

City

Egypt, kafrelsheikh government

Orcid

-

Volume

71

Article Issue

184

Related Issue

52949

Issue Date

2025-01-01

Receive Date

2024-08-18

Publish Date

2025-01-01

Page Start

44

Page End

60

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_395854.html

Detail API

http://journals.ekb.eg?_action=service&article_code=395854

Order

395,854

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

PREVALENCE OF BACILLUS CEREUS AND DETECTION OF SOME ENTEROTOXIGENIC GENES WITH QUALITY EVALUATION IN SOME FAST-FOODS

Details

Type

Article

Created At

13 Jan 2025