This study was conducted on 100 Egyptian Baladi ewes including 80 pregnant ewes and 20 post-parturient ewes. The aim of this study was to evaluate the biochemical and hormonal parameters in ewes during early, mid, late pregnancy and post-partum condition as well as in the single and twin pregnancies. Additionally, the impact of age and shearing on inflammatory and stress biomarkers were assessed. Results showed a significant decrease in IL-6 with significant increase of Cortisol in mid and late stage of pregnancy. In addition, significant increase in GPx during late pregnancy, there was a significant decrease in serum Sodium at mid and late pregnancy, while there was significant increase in Creatinine at late pregnancy compared to early pregnancy In addition; there was significant decrease in urea in mid and late pregnancy compared to the post-partum stage. Serum AST and ALT were significantly increased in late pregnancy compared to other groups. The mean values of Gpx and cortisol were significantly increased in ewes carrying twins compared to single fetus. Furthermore, significant differences were found between young and senile ewes in the mean values of IL-6, cortisol and urea, with increases observed in pregnant senile ewes. Moreover, the study revealed significant increase pregnant ewes after shearing in the mean values of IL2, IL6, cortisol, Gpx, and leptin. In conclusion, the study shows that biochemical and hormonal profiles of ewes are significantly impacted by pregnancy stage, litter size, age and shearing. Therefore, improved sheep management and health monitoring are required.