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402596

Effect of soy lecithin and green tea extract supplementation in Tris-based extender on cryopreserved buffalo bull semen quality

Article

Last updated: 07 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Theriogenology

Abstract

This study aimed to investigate the effect of soy lecithin (SL) or soy lecithin nanoparticles (nano-SL) together with green tea extract (GTE) on the frozen-thawed buffalo bull semen quality through its incorporation in the extended semen before cryopreservation. Semen was collected once weekly for six consecutive weeks from twelve Egyptian buffalo bulls of proven fertility (n = 72 samples), using a bovine artificial vagina. Semen was divided into 3 aliquots, each was extended with one of three extenders: Extender 1 (20% egg yolk-Tris), Extender 2 (4% SL+ 0.75% GTE-Tris), and Extender 3 (2% nano-SL+0.75% GTE-Tris), before cooling, equilibration, and freezing procedures. The frozen-thawed semen was assessed for motility and kinematics, acrosomal intactness, plasma membrane and DNA integrities, and antioxidant properties. The results showed that the extenders 2 and 3 considerably (P< 0.0001) improved sperm motility (total and progressive motilities), DNA intactness, and improved the semen antioxidant properties (increase the total antioxidant capacity, superoxide dismutase, catalase, reduced glutathione activities, and decreased malondialdehyde levels). In conclusion, the incorporation of soy lecithin or soy lecithin nanoparticles together with green tea extract in semen extenders improved the frozen-thawed buffalo bull semen quality.

DOI

10.21608/bvmj.2024.338058.1895

Keywords

Keywords: Antioxidant markers, Buffalo bull, frozen semen, Green tea extract, Soy lecithin nanoparticles

Authors

First Name

asmaa

Last Name

ghania

MiddleName

salah

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.

Email

asmaasalah854@gmail.com

City

El- Qalibua

Orcid

-

First Name

Alaa

Last Name

Abdelghafar

MiddleName

Elsayed

Affiliation

1Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. 2Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa, Dakahlia, Egypt.

Email

abdel_ghafar@yahoo.com

City

El- Qalibua

Orcid

-

First Name

Gamal

Last Name

Sosa

MiddleName

Abdel-raheem Mohamed

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.

Email

gamal_sosa@yahoo.com

City

Moshtohor-Tokh

Orcid

-

First Name

Magdy

Last Name

Badr

MiddleName

Ramadan

Affiliation

Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, Egypt.

Email

magdybadr69@yahoo.com

City

-

Orcid

-

First Name

Mohsen

Last Name

agag

MiddleName

Abd Elhafeez

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.

Email

mohsenagag@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Kandiel

MiddleName

M. M.

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.

Email

kandil175@hotmail.com

City

-

Orcid

-

Volume

47

Article Issue

2

Related Issue

52736

Issue Date

2024-12-01

Receive Date

2024-11-20

Publish Date

2024-12-31

Page Start

52

Page End

57

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 47_Issue 2_Pages 52-57.pdf

PDF . 387.4kB

Link

https://bvmj.journals.ekb.eg/article_402596.html

Detail API

http://journals.ekb.eg?_action=service&article_code=402596

Order

402,596

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Effect of soy lecithin and green tea extract supplementation in Tris-based extender on cryopreserved buffalo bull semen quality

Details

Type

Article

Created At

07 Jan 2025