Soaking soybean seeds (Glycne Max. L Essex variety) in different solutions such as deionized water, acetic acid (from 0.03-0.6N), sodium bicarbonate and sodium hydroxide (0.01-0.1 N) for 6,12 and 24 hr., reduced the antinutritional factors. Soaking soybean seeds in deionized water, acetic acid, Sodium bicarbonate and Sodium hydroxide for 24hr. reduced trypsin inhibitor activities by (57.0, 53.0, 70.6, 100%), phytic acid (32.69, 42.31, 36.54, 42.31%), lipoxygenase (33.41, 84.80, 85.40, 96.0%), respectively.